Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Mouse Cell lysate - whole cell (Liver tissue)
Specification
Liver tissue
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5%BSA
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: Rabbit-HRP
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/4000
Detection
Detection method
West Pico
Bands
Specific: 20 kDa Non-specific: 35, 50 kDa
Exposure
1 minute(s) and 0 second(s)
Additional data
Abcam response
Thank you for your review. I am sorry you experienced difficulty using this antibody. To decrease the background bands you may want to reduce the incubation time to 2 hours at room temperature rather than doing an overnight incubation. Please be advised this product is backed by our Abpromise guarantee for use in mouse WB, and we can provide a replacement antibody, credit or refund provided the purchase was within the past 12 months.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Suypriya Sen
Verified customer
投稿 May 13 2014