Application
Flow Cytometry
Sample
Mouse Cell (EL4 T cells tumor line and primary hepatocytes)
Permeabilization
No
Gating Strategy
Positive cells for FITC
Specification
EL4 T cells tumor line and primary hepatocytes
Preparation
Cell harvesting/tissue preparation method: Mice were dissected to obtain the liver. The liver was then mashed to obtain hepatocytes.
Sample buffer: PBS with 10% bovine serum
Sample buffer: PBS with 10% bovine serum
Fixation
none
Other product details
Incubation time
20 minute(s) · Temperature: 4°C · Diluent: PBS with 10% bovine serum
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabbit IgG adsorbed to FITC
Host species: Goat
Clonality: Monoclonal
Conjugation: Fluorescein
Host species: Goat
Clonality: Monoclonal
Conjugation: Fluorescein
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Safa Mohamad
Verified customer
投稿 May 12 2016