Application
Western blot
Sample
Mouse Tissue lysate - whole (Organ, 293 cell, C2C12 cell)
Loading amount
25 µg
Specification
Organ, 293 cell, C2C12 cell
Gel Running Conditions
Reduced Denaturing (10% Tris-Tricine gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Skim milk/TBST
Secondary antibody
Name
Non-Abcam antibody was used: Anti-rabbit IgG, HRP-linked Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
Immunostar LD
Exposure
30 second(s)
Bands
Specific: about 9 kDa Non-specific: many kDa
Positive control
Liver
Additional data
Additional Notes
lane1: Lung
lane2: Spleen
lane3: Testis
lane4: Tibialis anterior muscle
lane5: Quadriceps femoris muscle
lane6: Heart
lane7: Brain
lane8: Liver
lane9: 293 cell lysate
lane10: C2C12 cell lysate
lane2: Spleen
lane3: Testis
lane4: Tibialis anterior muscle
lane5: Quadriceps femoris muscle
lane6: Heart
lane7: Brain
lane8: Liver
lane9: 293 cell lysate
lane10: C2C12 cell lysate
Abcam response
Thank you for your honest feedback. This antibody has only been tested against the recombinant protein and may not be suitable for the endogenous protein.
The customer also submitted an image with this review, but unfortunately, it has gone missing during our digital croping process. We apologize for this.
The customer also submitted an image with this review, but unfortunately, it has gone missing during our digital croping process. We apologize for this.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Oct 19 2010