Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain, liver)
Loading amount
30 µg
Specification
Brain, liver
Gel Running Conditions
Reduced Denaturing (9%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/1000
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Secondary antibody
Name
Non-Abcam antibody was used: Santa cruz biotechnology, anti rabbit IgG HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
Chemiluminescent
Exposure
20 second(s)
Bands
Specific: 135 kDa Non-specific: 135 kDa
Positive control
WT brain, liver
Negative control
IFNAR1 -/-, brain, liver
Additional data
Additional Notes
ABTRIAL-EBITS
Abcam response
This customer received an AbTrial discount to test this antibody in WB, an untested application. These preliminary data suggest that this antibody may not be suitable for this application, however further testing is necessary.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Thomas Blank
Verified customer
投稿 Apr 23 2013