Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Spleen)
Specification
Spleen
Fixative
Acetone
Permeabilization
No
Blocking step
Casein as blocking agent for 15 minute(s) · Concentration: 0.25% · Temperature: RT°C
Other product details
Dilution
1/50
Incubation time
1 hour(s) and 0 minute(s) · Diluent: BSA in TBS
Secondary antibody
Name
Non-Abcam antibody was used: Envision Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
The antibody did not seem to stain frozen mouse tissue. The protocols tested included high dilutions of primary as well as methanol:acetone 1:1 fixation. The antibody just does not appear to stain fresh frozen mouse tissue.
Abcam response
We would like to thank the reviewer for submitting this Abreview for this product. As the product had not yet been tested in this application on this tissue type we were unsure what the result might be and we appreciate the time and effort that the reviewer has put in to testing this product in this application. The customer has received a free primary antibody of their choice for performing these tests and submitting this Abreview. We wish to thank them for participating in our 100% Abreview testing discount program.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 May 29 2012