Anti-ICAM1 抗体 [15.2] (ab20)
Key features and details
- Mouse monoclonal [15.2] to ICAM1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-ICAM1 antibody [15.2]
ICAM1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [15.2] to ICAM1 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details
適用なし: IHC-P -
種交差性
交差種: Human -
免疫原
Tissue, cells or virus corresponding to Human ICAM1. Human monocytes.
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ポジティブ・コントロール
- WB: SK N BE, Saos-2 and HT1080 whole cell lysate. Flow Cytometry: HK-2 cells stimulated with TGF-beta1. IHC: Human amniotic membrane tissues.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. Store undiluted. -
バッファー
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.14% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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精製度
Affinity purified -
特記事項(精製)
This purified format is guaranteed to be >90% pure as determined by SDS-PAGE analysis. -
ポリ/モノ
モノクローナル -
クローン名
15.2 -
ミエローマ
Sp2/0 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab20の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 58 kDa.
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特記事項 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 58 kDa. |
ターゲット情報
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機能
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus. -
配列類似性
Belongs to the immunoglobulin superfamily. ICAM family.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains. -
翻訳後修飾
Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3383 Human
- Omim: 147840 Human
- SwissProt: P05362 Human
- Unigene: 643447 Human
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別名
- Antigen identified by monoclonal antibody BB2 antibody
- BB 2 antibody
- BB2 antibody
see all
画像
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All lanes : Anti-ICAM1 antibody [15.2] (ab20) at 5 µg/ml
Lane 1 : SK N BE (Human neuroblastoma) whole cell lysate
Lane 2 : Saos-2 (Human epithelial-like osteosarcoma cell line) whole cell lysate
Lane 3 : HT1080 (Human fibrosarcoma) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 58 kDa
Observed band size: 58 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (26)
ab20 は 26 報の論文で使用されています。
- Ma L et al. Agrin Influences Botulinum Neurotoxin A-Induced Nerve Sprouting via miR-144-agrin-MuSK Signaling. Front Cell Dev Biol 8:15 (2020). PubMed: 32083076
- Bernabeu M et al. Binding Heterogeneity of Plasmodium falciparum to Engineered 3D Brain Microvessels Is Mediated by EPCR and ICAM-1. mBio 10:N/A (2019). PubMed: 31138740
- Wu D et al. The Effects of the CXCR4 Antagonist, AMD3465, on Human Retinal Vascular Endothelial Cells (hRVECs) in a High Glucose Model of Diabetic Retinopathy. Med Sci Monit 25:6946-6954 (2019). PubMed: 31860633
- Iwasaki O et al. Involvement of condensin in cellular senescence through gene regulation and compartmental reorganization. Nat Commun 10:5688 (2019). PubMed: 31831736
- Xu L et al. Peroxisome Proliferator-Activated Receptor-? Antagonizes LOX-1-Mediated Endothelial Injury by Transcriptional Activation of miR-590-5p. PPAR Res 2019:2715176 (2019). PubMed: 31354796