Hydroxymethylated DNA Quantification Kit (Colorimetric) (ab117130)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 3 hr 45 min
- Sample type: Adherent cells, Plasma, Saliva, Serum, Suspension cells, Tissue, Urine
- Sensitivity: 40 pg/well
製品の概要
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製品名
Hydroxymethylated DNA Quantification Kit (Colorimetric)
hydroxymethylated DNA キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Saliva, Urine, Serum, Plasma, Tissue, Adherent cells, Suspension cells -
アッセイタイプ
Quantitative -
検出感度
>= 40 pg/well -
全工程の試験時間
3h 45m -
製品の概要
DNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in 5-methylcytosine (5-mC). In somatic cells, 5-mC is found almost exclusively in the context of paired symmetrical methylation of the dinucleotide CpG. The biological important of 5-mC as a major epigenetic modification in phenotype and gene expression has been recognized widely. Quite recently, a novel modified nucleotide, 5-hydroxymethylcytosine (5-hmC), has been detected to be abundant in mouse brain and embryonic stem cells. It is a hydroxylated and methylated form of cytosine. Although it is still uncertain its specific role in epigenetics, it is believed it plays a role in DNA demethylation, chromatin remodeling and gene expression regulation.
Abcam's Hydroxymethylated DNA Quantification Kit (Colorimetric) (ab117130) is a complete set of optimized buffers and reagents to quantify global DNA hydroxymethylation by specifically measuring levels of 5-hydroxymethylcytosine (5-hmC) without cross-reactivity to methylcytosine and unmethylated cytosine.
It can be paired to use with Methylated DNA Quantification Kit (Colorimetric) (ab117128), which specifically quantifies 5-mC, for simultaneously quantification of both methylated and hydroxymethylated DNA.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Please refer to protocols. -
内容 48 tests 96 tests 10X Wash Buffer 1 x 14ml 1 x 28ml 8-Well Assay Strips (with Frame) 6 units 12 units Binding Solution 1 x 5ml 1 x 10ml Capture Antibody, 1000 µg/mL 1 x 4µl 1 x 8µl Detection Antibody, 400 µg/mL 1 x 8µl 1 x 16µl Developer Solution 1 x 5ml 1 x 10ml Enhancer Solution 1 x 8µl 1 x 16µl Negative Control I, 20 µg/mL 1 x 10µl 1 x 20µl Negative Control II, 20 µg/mL 1 x 10µl 1 x 20µl Positive Control, 20 µg/mL 1 x 10µl 1 x 20µl Stop Solution 1 x 5ml 1 x 10ml -
研究分野
関連製品
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Related Products
画像
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Demonstration of a high abundance of 5-hydroxymethylcytosine in human brain and colon tissues. 200 ng of DNA isolated from different tissues or cells were added into the assay wells and the hydroxymethylated fraction of DNA was measured using the ab117130. OD intensity measured in variety of DNA samples.
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Demonstration of high specificity of 5-hydroxymethylcytosine detection achieved using ab117130. Synthetic unmethylated DNA (contains only cytosine), methylated DNA (contains only 5-methylcytosine), and hydroxymethylated DNA standard (contains only 5-hydroxymethylcytosine) were added into the assay wells at different concentrations and then measured with ab117130.
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Demonstration of a high abundance of 5-hydroxymethylcytosine in human brain and colon tissues. 200 ng of DNA isolated from different tissues or cells were added into the assay wells and the hydroxymethylated fraction of DNA was measured using ab117130. Percentage of hydroxymethylated DNA in various tissues and cells.
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Demonstration of high sensitivity of 5-hydroxymethylcytosine detection achieved using ab117130. Synthetic hydroxymethylated DNA was added into the assay wells at different concentrations and then measured with the ab117130.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab117130 は 5 報の論文で使用されています。
- Ismail A et al. Early Development of Cardiac Fibrosis in Young Old-Father Offspring. Oxid Med Cell Longev 2022:8770136 (2022). PubMed: 36193084
- Ganguly K et al. Addressing the challenges of E-cigarette safety profiling by assessment of pulmonary toxicological response in bronchial and alveolar mucosa models. Sci Rep 10:20460 (2020). PubMed: 33235237
- Raad G et al. Adverse effects of paternal obesity on the motile spermatozoa quality. PLoS One 14:e0211837 (2019). PubMed: 30742661
- Thakur C et al. Loss of mdig expression enhances DNA and histone methylation and metastasis of aggressive breast cancer. Signal Transduct Target Ther 3:25 (2018). PubMed: 30254753
- Pérez LM et al. Altered metabolic and stemness capacity of adipose tissue-derived stem cells from obese mouse and human. PLoS One 10:e0123397 (2015). PubMed: 25875023