Application
Western blot
Sample
Human Recombinant protein (Human recombinant hPL)
Loading amount
0.1 µg
Specification
Human recombinant hPL
Blocking step
Milk as blocking agent for 17 hour(s) and 0 minute(s) · Concentration: 5%
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Amersham ECL Anti-mouse IgG whole antibody
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
20 minute(s) and 0 second(s)
Positive control
Western blot of the same membrane with anti-hPL antibody ab15554
Negative control
no
Additional data
Additional Notes
Although the antibody does not work for Western blot, it might be used for indirect immunofluorescence of hPL in human cells
Abcam response
This antibody has been tested in ICC/IF and not in WB. The data presented here suggest that ab11396 is not suited for use in WB and that ab15554 is a very good alternative.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Stoil Dmitrov
Verified customer
投稿 Apr 16 2007