Human beta 2 Microglobulin ELISA Kit (B2M) (ab99977)
Key features and details
- Sensitivity: 6 pg/ml
- Range: 15.6 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human beta 2 Microglobulin ELISA Kit (B2M)
beta 2 Microglobulin キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 6 pg/ml -
検出範囲
15.6 pg/ml - 1000 pg/ml -
添加回収試験
< 100 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 104.6 93% - 118% Serum 94.28 80% - 103% Plasma 95.78 81% - 105% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s beta 2 Microglobulin (B2M) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human beta 2 Microglobulin in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human beta 2 Microglobulin coated on a 96-well plate. Standards and samples are pipetted into the wells and beta 2 Microglobulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human beta 2 Microglobulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of beta 2 Microglobulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get higher sensitivity in only 90 minutes with Human Beta-2-Microglobulin ELISA Kit (ab181423) from our SimpleStep ELISA® range.
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特記事項
Optimisation may be required with urine samples.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 2 x 30ml beta 2 Microglobulin Microplate (12 x 8 wells) 1 unit Biotinylated anti-Human beta 2 Microglobulin 2 vials Recombinant Human beta 2 Microglobulin Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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機能
Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. -
関連疾患
Defects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) [MIM:241600]. Affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation.
Note=Beta-2-microglobulin may adopt the fibrillar configuration of amyloid in certain pathologic states. The capacity to assemble into amyloid fibrils is concentration dependent. Persistently high beta(2)-microglobulin serum levels lead to amyloidosis in patients on long-term hemodialysis. -
配列類似性
Belongs to the beta-2-microglobulin family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain. -
翻訳後修飾
Glycation of Ile-21 is observed in long-term hemodialysis patients. -
細胞内局在
Secreted. Detected in serum and urine. - Information by UniProt
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別名
- B2M
- B2MG_HUMAN
- Beta 2 microglobin
see all -
参照データベース
- Entrez Gene: 567 Human
- Omim: 109700 Human
- SwissProt: P61769 Human
- Unigene: 534255 Human
- Unigene: 702947 Human
関連製品
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SimpleStep ELISA kits
画像
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab99977 は 1 報の論文で使用されています。
- Lee SH et al. Proteome alterations in the aqueous humor reflect structural and functional phenotypes in patients with advanced normal-tension glaucoma. Sci Rep 12:1221 (2022). PubMed: 35075201