Human Transferrin ELISA Kit (ab108902)
Key features and details
- Sensitivity: 0.78 ng/ml
- Range: 6.25 ng/ml - 50 ng/ml
- Sample type: Cell culture supernatant, Cell Lysate, Milk, Saliva, Tissue
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human Transferrin ELISA Kit
Transferrin キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Overall 4.5% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Overall 9.6% -
サンプルの種類
Cell culture supernatant, Saliva, Milk, Tissue, Cell Lysate -
アッセイタイプ
Sandwich (quantitative) -
検出感度
= 0.78 ng/ml -
検出範囲
6.25 ng/ml - 50 ng/ml -
添加回収試験
97 %
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全工程の試験時間
4h 00m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s Tranferrin Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Transferrin in milk, urine, saliva, CSF, cell culture, cell lysate, and tissue samples.
A Tranferrin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Tranferrin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Tranferrin captured in plate.
Get results in 90 minutes with Human Transferrin ELISA Kit (ab187391) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Transferrin Antibody 1 x 120µl Chromogen Substrate 1 x 7ml Sealing Tapes 3 units Stop Solution 1 x 11ml Transferrin Microplate (12 x 8 well strips) 1 unit Transferrin Standard 1 vial -
研究分野
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機能
Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. -
組織特異性
Expressed by the liver and secreted in plasma. -
関連疾患
Defects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia. -
配列類似性
Belongs to the transferrin family.
Contains 2 transferrin-like domains. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Apotransferrin
- Beta 1 metal binding globulin
- Beta-1 metal-binding globulin
see all -
参照データベース
- Entrez Gene: 7018 Human
- Omim: 190000 Human
- SwissProt: P02787 Human
- Unigene: 518267 Human
関連製品
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SimpleStep ELISA kits
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab108902 は 4 報の論文で使用されています。
- Kaur H et al. Higher CSF Ferritin Heavy-Chain (Fth1) and Transferrin Predict Better Neurocognitive Performance in People with HIV. Mol Neurobiol 58:4842-4855 (2021). PubMed: 34195939
- Hazlett HF et al. Altered iron metabolism in cystic fibrosis macrophages: the impact of CFTR modulators and implications for Pseudomonas aeruginosa survival. Sci Rep 10:10935 (2020). PubMed: 32616918
- Mehta SR et al. Cerebrospinal fluid cell-free mitochondrial DNA is associated with HIV replication, iron transport, and mild HIV-associated neurocognitive impairment. J Neuroinflammation 14:72 (2017). ELISA ; Human . PubMed: 28359324
- Patton SM et al. Cerebrospinal fluid (CSF) biomarkers of iron status are associated with CSF viral load, antiretroviral therapy, and demographic factors in HIV-infected adults. Fluids Barriers CNS 14:11 (2017). ELISA ; Human . PubMed: 28427421