Human TACE ELISA Kit (ADAM17) (ab113321)
Key features and details
- Sensitivity: 70 pg/ml
- Range: 78.15 pg/ml - 5000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human TACE ELISA Kit (ADAM17) -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 70 pg/ml -
検出範囲
78.15 pg/ml - 5000 pg/ml -
添加回収試験
100 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 98.65 89% - 124% Serum 112.3 85% - 132% Plasma 101.1 72% - 128% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s TACE Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TACE in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human TACE coated on a 96-well plate. Standards and samples are pipetted into the wells and TACE present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TACE antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TACE bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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特記事項
Optimization may be required with urine samples.
The standard is affinity purified.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 ラベル 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated anti-Human TACE Item F 2 vials Recombinant Human TACE Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TACE Microplate (12 x 8 wells) Item A 1 unit TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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参照データベース
- SwissProt: P78536 Human
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab113321 は 1 報の論文で使用されています。
- Erdoes G et al. CD62L (L-selectin) shedding for assessment of perioperative immune sensitivity in patients undergoing cardiac surgery with cardiopulmonary bypass. PLoS One 8:e53045 (2013). ELISA ; Human . PubMed: 23301018