Human Syndecan-1 ELISA Kit (CD138) (ab46506)
Key features and details
- Sensitivity: 4.94 ng/ml
- Range: 8 ng/ml - 256 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human Syndecan-1 ELISA Kit (CD138)
Syndecan-1 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Overall 6 6.2% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Overall 6 10.2% -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
= 4.94 ng/ml -
検出範囲
8 ng/ml - 256 ng/ml -
添加回収試験
101 %
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全工程の試験時間
1h 45m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Human Syndecan-1 ELISA (CD138) kit is designed for the quantitative measurement of Syndecan-1 in Human serum, plasma, buffered solutions and supernatants.
A monoclonal antibody specific for Syndecan-1 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known Syndecan-1 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for Syndecan-1 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of Syndecan-1 present in the samples.
This kit will recognize both endogenous and recombinant Human Syndecan-1.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 ラベル 1 x 96 tests 2 x 96 tests 1 x 96 tests 2 x 96 tests 10X Standard Diluent Buffer Black 1 x 15ml 1 x 25ml 1 x 15ml 1 x 25ml 200X Wash Buffer White 1 x 10ml 2 x 10ml 1 x 10ml 2 x 10ml Biotinylated Antibody Diluent Red 1 x 7ml 1 x 13ml 1 x 7ml 1 x 13ml Biotinylated anti-Syndecan-1 Red 1 x 400µl 2 x 400µl 1 x 400µl 2 x 400µl Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml 1 x 11ml 1 x 24ml Control Yellow 2 vials 4 vials 2 vials 4 vials HRP Diluent Red 1 x 12ml 1 x 23ml 1 x 12ml 1 x 23ml Plastic Plate Covers 2 units 4 units 2 units 4 units Stop Reagent Black 1 x 11ml 2 x 11ml 1 x 11ml 2 x 11ml Streptavidin-HRP 2 x 5µl 4 x 5µl 2 x 5µl 4 x 5µl Syndecan-1 Microplate (12 x 8 well strips) 1 unit 2 units 1 unit 2 units Syndecan-1 Standard (lyophilized) Orange 2 vials 4 vials 2 vials 4 vials -
研究分野
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機能
Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix. -
配列類似性
Belongs to the syndecan proteoglycan family. -
細胞内局在
Membrane. - Information by UniProt
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別名
- CD_antigen
- CD138
- CD138 antigen
see all -
参照データベース
- Entrez Gene: 6382 Human
- Omim: 186355 Human
- SwissProt: P18827 Human
- Unigene: 224607 Human
- Unigene: 665958 Human
画像
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Syndecan measured in undiluted cell culture supernatants, including the positive control from kit (duplicates +/- SD).
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Syndecan measured in human biological fluids (dilution range 1/1-1/10); mouse and pig samples (plasma and serum) were below limit of detection (duplicates +/- SD).
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Standard curve of human syndecan with background signal subtracted (duplicates; +/- SD).
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Representative Standard Curve using ab46506
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (26)
ab46506 は 26 報の論文で使用されています。
- Tunjungputri RN et al. Effect of oseltamivir phosphate versus placebo on platelet recovery and plasma leakage in adults with dengue and thrombocytopenia; a phase 2, multicenter, double-blind, randomized trial. PLoS Negl Trop Dis 16:e0010051 (2022). PubMed: 34995275
- Bauer C et al. Minimal Change Disease Is Associated With Endothelial Glycocalyx Degradation and Endothelial Activation. Kidney Int Rep 7:797-809 (2022). PubMed: 35497798
- Yin T et al. Dynamics of intracranial and peripheral plasma Syndecan-1 after ischemic stroke with large vessel occlusion. CNS Neurosci Ther 28:1648-1650 (2022). PubMed: 35790077
- Yu WK et al. Angiopoietin-2 outperforms other endothelial biomarkers associated with severe acute kidney injury in patients with severe sepsis and respiratory failure. Crit Care 25:48 (2021). PubMed: 33541396
- Stojanovic D et al. The Discriminatory Ability of Renalase and Biomarkers of Cardiac Remodeling for the Prediction of Ischemia in Chronic Heart Failure Patients With the Regard to the Ejection Fraction. Front Cardiovasc Med 8:691513 (2021). PubMed: 34395559