Human SCF ELISA Kit (Kit Ligand) (ab108901)
Key features and details
- Sensitivity: 0.11 ng/ml
- Range: 0.156 ng/ml - 40 ng/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human SCF ELISA Kit (Kit Ligand)
SCF キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Overall 4.8% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Overall 9.2% -
サンプルの種類
Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
= 0.11 ng/ml -
検出範囲
0.156 ng/ml - 40 ng/ml -
添加回収試験
95 %
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全工程の試験時間
4h 00m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s Stem Cell Factor (Kit Ligand) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Stem Cell Factor concentrations in Human plasma, serum and cell culture supernatants.
A Stem Cell Factor specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Stem Cell Factor specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Stem Cell Factor captured in plate.
Store kit at 4°C immediately upon receipt, apart from the Streptavidin-Peroxidase Conjugate & Biotinylated Antibody, which should be stored at -20°C.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 20ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Stem Cell Factor Antibody 1 x 120µl Chromogen Substrate 1 x 7ml Sealing Tapes 3 units Stem Cell Factor Microplate (12 x 8 well strips) 1 unit Stem Cell Factor Standard 1 vial Stop Solution 1 x 11ml -
研究分野
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機能
Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins. -
関連疾患
Hyperpigmentation with or without hypopigmentation, familial progressive
Deafness, congenital, unilateral or asymmetric -
配列類似性
Belongs to the SCF family. -
発生段階
Acts in the early stages of hematopoiesis. -
翻訳後修飾
A soluble form (sKITLG) is produced by proteolytic processing of isoform 1 in the extracellular domain.
Found in two differentially glycosylated forms, LMW-SCF and HMW-SCF. LMW-SCF is fully N-glycosylated at Asn-145, partially N-glycosylated at Asn-90, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97 or Asn-118. HMW-SCF is N-glycosylated at Asn-118, Asn-90 and Asn-145, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97.
A soluble form exists as a cleavage product of the extracellular domain. -
細胞内局在
Secreted; Cell membrane and Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane. Cell projection, lamellipodium. Cell projection, filopodium. - Information by UniProt
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別名
- C kit ligand
- C-kit ligand
- Ckit ligand
see all -
参照データベース
- Entrez Gene: 4254 Human
- Omim: 184745 Human
- SwissProt: P21583 Human
- Unigene: 1048 Human
- Unigene: 661108 Human
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab108901 は 3 報の論文で使用されています。
- Kefayat A et al. Diagnostic and prognostic value of stem cell factor plasma level in glioblastoma multiforme patients. Cancer Med 10:5154-5162 (2021). PubMed: 34250760
- Li G et al. Human limbal niche cells are a powerful regenerative source for the prevention of limbal stem cell deficiency in a rabbit model. Sci Rep 8:6566 (2018). PubMed: 29700361
- Lupicka M et al. Identification of pluripotent cells in bovine uterus: in situ and in vitro studies. Reproduction 149:317-27 (2015). Sandwich ELISA . PubMed: 25556182