Human Procalcitonin ELISA Kit (ab100630)
Key features and details
- Sensitivity: 20 pg/ml
- Range: 27.43 pg/ml - 20000 pg/ml
- Sample type: Cell culture supernatant, Plasma
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human Procalcitonin ELISA Kit -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 20 pg/ml -
検出範囲
27.43 pg/ml - 20000 pg/ml -
添加回収試験
83 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 100.5 80% - 111% Plasma 72.35 65% - 88% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s Procalcitonin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Procalcitonin in plasma and cell culture supernatants. (Human Procalcitonin concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for Human Procalcitonin coated on a 96-well plate. Standards and samples are pipetted into the wells and Procalcitonin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Procalcitonin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Procalcitonin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect endogenous Human Procalcitonin in normal serum with ab100630, only in serum spiked with Human Procalcitonin.
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特記事項
Optimization may be required with urine samples.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml 700X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated anti-Human Procalcitonin 2 vials Procalcitonin Microplate (12 x 8 wells) 1 unit Recombinant Human Procalcitonin Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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参照データベース
- Entrez Gene: 796 Human
- SwissProt: P01258 Human
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab100630 は 7 報の論文で使用されています。
- Mohany KM et al. Circulating miR-15b, Annexin A1, procalcitonin and interleukin-6 levels differentiate children with metabolically unhealthy obesity from those with metabolically healthy obesity: A case-control study. Exp Ther Med 23:403 (2022). PubMed: 35637648
- Rosado-Franco JJ et al. Recombinant Fasciola hepatica Fatty Acid Binding Protein as a Novel Anti-Inflammatory Biotherapeutic Drug in an Acute Gram-Negative Nonhuman Primate Sepsis Model. Microbiol Spectr 9:e0191021 (2021). PubMed: 34937173
- Rosado-Franco JJ et al. Outlining key inflammation-associated parameters during early phase of an experimental gram-negative sepsis model in rhesus macaques (Macaca mulatta). Animal Model Exp Med 2:326-333 (2019). PubMed: 31942564
- Ingram JR et al. Levels of wound calprotectin and other inflammatory biomarkers aid in deciding which patients with a diabetic foot ulcer need antibiotic therapy (INDUCE study). Diabet Med 35:255-261 (2018). PubMed: 28734103
- Wan Y et al. Adjuvant rhubarb alleviates organs dysfunction and inhibits inflammation in heat stroke. Exp Ther Med 16:1493-1498 (2018). PubMed: 30116399
- Wan B et al. Rhubarb vs. glycerin enema for treatment of critically ill patients with intra-abdominal hypertension. Exp Ther Med 14:855-861 (2017). PubMed: 28673010
- Nakahira K et al. Circulating mitochondrial DNA in patients in the ICU as a marker of mortality: derivation and validation. PLoS Med 10:e1001577; discussion e1001577 (2013). PubMed: 24391478