Human Mesenchymal Stromal Cell Marker Panel (CD44, CD45, CD90 CD29 and CD105) (ab93758)

  Immunocytochemistry/ Immunofluorescence - Human Mesenchymal Stromal Cell Marker Panel (CD44, CD45, CD90 CD29 and CD105) (ab93758)

ICC/IF image of ab52971 staining CD29 in Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52971, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  Flow Cytometry - Human Mesenchymal Stromal Cell Marker Panel (CD44, CD45, CD90 CD29 and CD105) (ab93758)

Asynchronous KM-H2 cells were pelleted and labeled by indirect immunofluorescence. Cells were stained with ab10559 (1/200) for 30min at 4'C, washed and then stained with goat anti-rabbit alexafluor 488 (1/200). Forward/Side scatter were used to eliminate cellular debris. The accompanying marker was applied such that only 2% of the IgG control was positive. Based on the accompanying image, approximately 9.62% of cells exhibited positive staining for anti-CD45. Since KM-H2 have low levels of CD45 transcripts, it is expected that they have low levels of CD45 on their surface which is reflected in the ~9% positive. This image is taken from an Abreview.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Human Mesenchymal Stromal Cell Marker Panel (CD44, CD45, CD90 CD29 and CD105) (ab93758)

Ab6124 staining CD44 on Human Tissue sections (Skin tumor) by IHC-P. Sections were Formaldehyde fixed and subjected to heat mediated antigen retrieval in citric acid prior to blocking with 10% serum for 30 mins at 25°C. The primary antibody was diluted 1/100 in PBS and incubated with the sample overnight at 4°C. A TRITC non-Abcam secondary antibody was used.