Human IL-6 ELISA Kit High Sensitivity (ab46042)
Key features and details
- Sensitivity: 0.8 pg/ml
- Range: 1.56 pg/ml - 50 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human IL-6 ELISA Kit High Sensitivity
IL-6 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Overall 4.4% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Sample 1 18 25pg/ml 3 12.2% Sample 2 18 12pg/ml 1 8.7% Sample 3 18 6pg/ml 1 13.7% Sample 4 18 41pg/ml 2 5.9% Sample 5 18 31pg/ml 1 4.9% -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 0.8 pg/ml -
検出範囲
1.56 pg/ml - 50 pg/ml -
添加回収試験
93 %
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全工程の試験時間
3h 45m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Human IL-6 ELISA kit - High sensitivity is a sandwich ELISA kit for the quantitative measurement of IL-6 in supernatants, buffered solutions, serum, plasma and other body fluids. This assay will recognise both natural and recombinant Human IL-6.
A monoclonal antibody specific for IL-6 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-6 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-6 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-6 present in the samples.
This kit will recognize both endogenous and recombinant Human IL-6.
Get results in 90 minutes with Human IL-6 ELISA Kit (ab178013) from our SimpleStep ELISA® range.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 ラベル 2 x 96 tests 1 x 96 tests 1 x 96 tests 10X Standard Diluent Buffer Black 1 x 25ml 1 x 15ml 1 x 15ml 200X Wash Buffer White 2 x 10ml 1 x 10ml 1 x 10ml Biotinylated Antibody Diluent Red 1 x 13ml 1 x 7.5ml 1 x 7.5ml Biotinylated anti-IL-6 Red 2 x 0.4ml 1 x 0.4ml 1 x 0.4ml Chromogen TMB Substrate Solution 1 x 24ml 1 x 11ml 1 x 11ml HRP Diluent Red 1 x 23ml 1 x 12ml 1 x 12ml IL-6 HS Control Yellow 4 vials 2 vials 2 vials IL-6 HS Standard (Lyophilized) Orange 4 vials 2 vials 2 vials IL-6 Microplate (12 x 8 well strips) 2 units 1 unit 1 unit Plastic Plate Covers 1 x 4 units 1 x 2 units 1 x 2 units Standard Diluent (Serum) 2 x 7ml 1 x 7ml 1 x 7ml Stop Reagent Black 2 x 11ml 1 x 11ml 1 x 11ml Streptavidin-HRP 4 x 5µl 2 x 5µl 2 x 5µl -
研究分野
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機能
Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. -
関連疾患
Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men. -
配列類似性
Belongs to the IL-6 superfamily. -
翻訳後修飾
N- and O-glycosylated. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Interleukin BSF 2
- B cell differentiation factor
- B cell stimulatory factor 2
see all -
参照データベース
- Entrez Gene: 3569 Human
- Omim: 147620 Human
- SwissProt: P05231 Human
- Unigene: 654458 Human
関連製品
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SimpleStep ELISA kits
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データシートおよび資料
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SDS download
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Datasheet download
参考文献 (67)
ab46042 は 67 報の論文で使用されています。
- Gómez-Rubio P et al. Salivary IL-6 Concentration Is Associated with Frailty Syndrome in Older Individuals. Diagnostics (Basel) 12:N/A (2022). PubMed: 35054284
- Liu C et al. Systematic analysis of IL-6 as a predictive biomarker and desensitizer of immunotherapy responses in patients with non-small cell lung cancer. BMC Med 20:187 (2022). PubMed: 35550592
- Anier K et al. Psychostimulant-induced aberrant DNA methylation in an in vitro model of human peripheral blood mononuclear cells. Clin Epigenetics 14:89 (2022). PubMed: 35842682
- Alegre-Martínez A et al. Plasma Nickel Levels Correlate with Low Muscular Strength and Renal Function Parameters in Patients with Prostate Cancer. Diseases 10:N/A (2022). PubMed: 35892733
- Ji J et al. lncRNA OIP5-AS1 knockdown or miR-223 overexpression can alleviate LPS-induced ALI/ARDS by interfering with miR-223/NLRP3-mediated pyroptosis. J Gene Med 24:e3385 (2022). PubMed: 34346534