Human CXCL1 ELISA Kit (GRO alpha) (ab100530)
Key features and details
- Sensitivity: 2 pg/ml
- Range: 2 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human CXCL1 ELISA Kit (GRO alpha)
CXCL1/GRO alpha キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
2 pg/ml -
検出範囲
2 pg/ml - 1000 pg/ml -
添加回収試験
> 99 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 112.2 101% - 122% Serum 130.4 119% - 145% Plasma 113.5 85% - 140% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s CXCL1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit (GRO alpha) is an in vitro enzyme designed for the quantitative measurement of Human GRO alpha in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human CXCL1 (GRO alpha) coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CXCL1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get higher sensitivity in only 90 minutes with Human CXCL1 ELISA Kit (GRO alpha) (ab190805) from our SimpleStep ELISA® range.
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特記事項
Optimization may be required with urine samples
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 20X Wash Buffer 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Biotinylated anti-Human GRO-alpha 2 vials GRO-alpha Microplate (12 x 8 wells) 1 unit Recombinant Human GRO-alpha Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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機能
Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity. -
配列類似性
Belongs to the intercrine alpha (chemokine CxC) family. -
翻訳後修飾
N-terminal processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) are produced by proteolytic cleavage after secretion from peripheral blood monocytes. -
細胞内局在
Secreted. - Information by UniProt
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別名
- C-X-C motif chemokine 1
- chemokine (C-X-C motif) ligand 1
- Chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
see all -
参照データベース
- Entrez Gene: 2919 Human
- Omim: 155730 Human
- SwissProt: P09341 Human
- Unigene: 708652 Human
- Unigene: 789 Human
関連製品
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SimpleStep ELISA kits
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab100530 は 2 報の論文で使用されています。
- Winkler MJ et al. Functional investigation of the coronary artery disease gene SVEP1. Basic Res Cardiol 115:67 (2020). PubMed: 33185739
- Li L et al. Increased ROS production in non-polarized mammary epithelial cells induces monocyte infiltration in 3D culture. J Cell Sci 130:190-202 (2017). PubMed: 27656113