Human Fetuin A ELISA Kit (AHSG) (ab108855)
Key features and details
- Sensitivity: 1.4 ng/ml
- Range: 3.125 ng/ml - 200 ng/ml
- Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
-
製品名
Human Fetuin A ELISA Kit (AHSG)
AHSG キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Overall 3.6% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Overall 8.7% -
サンプルの種類
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cerebral Spinal Fluid -
アッセイタイプ
Sandwich (quantitative) -
検出感度
1.4 ng/ml -
検出範囲
3.125 ng/ml - 200 ng/ml -
添加回収試験
98 %
-
全工程の試験時間
4h 00m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s Fetuin A (AHSG) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Fetuin A concentrations in Human plasma, serum, urine, milk and cell culture supernatants.
A Fetuin A specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Fetuin A specific biotinylated detection antibody is added and then followed by washing with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Fetuin A captured in plate.
Get higher sensitivity in only 90 minutes with Human Fetuin A ELISA Kit (AHSG) (ab185989) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
-
試験プラットフォーム
Microplate
製品の特性
-
保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Fetuin A Antibody 1 x 120µl Chromogen Substrate 1 x 7ml Fetuin A Microplate (12 x 8 well strips) 1 unit Fetuin A Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究分野
-
機能
Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Shows affinity for calcium and barium ions. -
組織特異性
Synthesized in liver and selectively concentrated in bone matrix. Secreted in plasma. It is also found in dentin in much higher quantities than other plasma proteins. -
配列類似性
Belongs to the fetuin family.
Contains 2 cystatin fetuin-A-type domains. -
翻訳後修飾
Phosphorylation sites are present in the extracelllular medium. -
細胞内局在
Secreted. - Information by UniProt
-
別名
- 59 kDa bone sialic acid-containing protein
- A2HS
- Aa2-066
see all -
参照データベース
- Entrez Gene: 197 Human
- Omim: 138680 Human
- SwissProt: P02765 Human
- Unigene: 324746 Human
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (7)
ab108855 は 7 報の論文で使用されています。
- Lin X et al. Plasma Exosomes Derived From Patients With End-Stage Renal Disease and Renal Transplant Recipients Have Different Effects on Vascular Calcification. Front Cell Dev Biol 8:618228 (2020). PubMed: 33585452
- Sieckmann T et al. Longitudinal studies of putative growth hormone (GH) biomarkers and hematological and steroidal parameters in relation to 2 weeks administration of human recombinant GH. Drug Test Anal 12:711-719 (2020). PubMed: 32115881
- Riedelová Z et al. SPR biosensor for quantification of fetuin-A as a promising multibiomarker. Physiol Res 67:S367-S375 (2018). PubMed: 30379557
- Niu R et al. iTRAQ-Based Proteomics Reveals Novel Biomarkers for Idiopathic Pulmonary Fibrosis. PLoS One 12:e0170741 (2017). ELISA ; Human . PubMed: 28122020
- Abdullah MI et al. Tissue and serum samples of patients with papillary thyroid cancer with and without benign background demonstrate different altered expression of proteins. PeerJ 4:e2450 (2016). Sandwich ELISA ; Human . PubMed: 27672505
- Májek P et al. Plasma proteome changes associated with refractory anemia and refractory anemia with ringed sideroblasts in patients with myelodysplastic syndrome. Proteome Sci 11:14 (2013). PubMed: 23566303
- Kuakarn S et al. Serum proteins in chronic hepatitis B patients treated with peginterferon alfa-2b. World J Gastroenterol 19:5067-75 (2013). Sandwich ELISA ; Human . PubMed: 23964140