Application
Other - Do not use
Predicted band size would be 62 kDa.
however observed band size for cow liver is less then 62 kDa.
Loading amount of each lane : 60ug of cow liver
1 lane: Fresh Primary antibody (ab41621)
2 lane: Fresh Primary antibody with 45 min incubation at RT
3 lane: only Secondary antibody (without primary antibody)
4 lane: Primary antibody : Peptide = 1 : 0.01with 45 min incubation at RT
5 lane: Primary antibody : Peptide = 1 : 0.001with 45 min incubation at RT
6 lane: Primary antibody : Peptide = 1 : 0.0001with 45 min incubation at RT
however observed band size for cow liver is less then 62 kDa.
Loading amount of each lane : 60ug of cow liver
1 lane: Fresh Primary antibody (ab41621)
2 lane: Fresh Primary antibody with 45 min incubation at RT
3 lane: only Secondary antibody (without primary antibody)
4 lane: Primary antibody : Peptide = 1 : 0.01with 45 min incubation at RT
5 lane: Primary antibody : Peptide = 1 : 0.001with 45 min incubation at RT
6 lane: Primary antibody : Peptide = 1 : 0.0001with 45 min incubation at RT
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Kwangwon Son
Verified customer
投稿 Nov 16 2017