Human CXCL7 ELISA Kit (ab100613)
Key features and details
- Sensitivity: 8.5 pg/ml
- Range: 4.1 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human CXCL7 ELISA Kit
CXCL7/PBP キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Serum, Plasma, Cell Lysate -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 8.5 pg/ml -
検出範囲
4.1 pg/ml - 1000 pg/ml -
添加回収試験
93 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 95.28 84% - 103% Serum 92.76 82% - 102% Plasma 93.45 83% - 103% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s CXCL7 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CXCL7 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human CXCL7 coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL7 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CXCL7 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL7 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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特記事項
Optimization may be required with urine samples.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human CXCL7 2 vials CXCL7 Microplate (12 x 8 wells) 1 unit Recombinant Human CXCL7 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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機能
LA-PF4 stimulates DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by human synovial cells. NAP-2 is a ligand for CXCR1 and CXCR2, and NAP-2, NAP-2(73), NAP-2(74), NAP-2(1-66), and most potent NAP-2(1-63) are chemoattractants and activators for neutrophils. TC-1 and TC-2 are antibacterial proteins, in vitro released from activated platelet alpha-granules. CTAP-III(1-81) is more potent than CTAP-III desensitize chemokine-induced neutrophil activation. -
配列類似性
Belongs to the intercrine alpha (chemokine CxC) family. -
翻訳後修飾
Proteolytic removal of residues 1-9 produces the active peptide connective tissue-activating peptide III (CTAP-III) (low-affinity platelet factor IV (LA-PF4)).
Proteolytic removal of residues 1-13 produces the active peptide beta-thromboglobulin, which is released from platelets along with platelet factor 4 and platelet-derived growth factor.
NAP-2(1-66) is produced by proteolytical processing, probably after secretion by leukocytes other than neutrophils.
NAP-2(73) and NAP-2(74) seem not be produced by proteolytical processing of secreted precursors but are released in an active form from platelets. -
細胞内局在
Secreted. - Information by UniProt
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別名
- B TG1
- Beta TG
- Beta thromboglobulin
see all -
参照データベース
- Entrez Gene: 5473 Human
- Omim: 121010 Human
- SwissProt: P02775 Human
- Unigene: 2164 Human
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データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab100613 は 4 報の論文で使用されています。
- Kollikowski AM et al. Platelet Activation and Chemokine Release Are Related to Local Neutrophil-Dominant Inflammation During Hyperacute Human Stroke. Transl Stroke Res 13:364-369 (2022). PubMed: 34455571
- Li C et al. Potential Markers from Serum-Purified Exosomes for Detecting Oral Squamous Cell Carcinoma Metastasis. Cancer Epidemiol Biomarkers Prev 28:1668-1681 (2019). PubMed: 31350263
- Du Q et al. CTAPIII/CXCL7: a novel biomarker for early diagnosis of lung cancer. Cancer Med 7:325-335 (2018). PubMed: 29356357
- Alsmadi NZ et al. Constricted microfluidic devices to study the effects of transient high shear exposure on platelets. Biomicrofluidics 11:064105 (2017). PubMed: 29204246