Human CCL28 ELISA Kit (ab99988)
Key features and details
- Sensitivity: 25 pg/ml
- Range: 27.4 pg/ml - 20000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human CCL28 ELISA Kit
CCL28/MEC キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 25 pg/ml -
検出範囲
27.4 pg/ml - 20000 pg/ml -
添加回収試験
> 65 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 67.7 59% - 81% Serum 70.6 60% - 98% Plasma 68.6 59% - 82% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s CCL28 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CCL28 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human CCL28 coated on a 96-well plate. Standards and samples are pipetted into the wells and CCL28 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated antiHuman CCL28 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CCL28 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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特記事項
Optimization may be required with urine samples.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human CCL28 2 vials CCL28 Microplate (12 strips x 8 wells) 1 x 200µl Recombinant Human CCL28 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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機能
Chemotactic activity for resting CD4, CD8 T-cells and eosinophils. Binds to CCR3 and CCR10 and induces calcium mobilization in a dose-dependent manner. -
組織特異性
Preferentially expressed by epithelial cells of diverse tissues including normal and pathological colon, salivary gland, mammary gland, trachea and rectum. Also found in prostate, spleen, thyroid, psoriasis skin and in lower levels in peripheral blood leukocytes, small intestine, Peyer patches, stomach and normal skin. -
配列類似性
Belongs to the intercrine beta (chemokine CC) family. -
細胞内局在
Secreted. - Information by UniProt
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別名
- C C motif chemokine ligand 28
- C-C motif chemokine 28
- CC chemokine CCL28
see all -
参照データベース
- Entrez Gene: 56477 Human
- Omim: 605240 Human
- SwissProt: Q9NRJ3 Human
- Unigene: 656904 Human
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab99988 は 2 報の論文で使用されています。
- Huang G et al. Hypoxia induced CCL28 promotes angiogenesis in lung adenocarcinoma by targeting CCR3 on endothelial cells. Sci Rep 6:27152 (2016). PubMed: 27250766
- Yoon JH et al. Plasma CC-chemokine ligand 28 level is correlated with hematopoietic stem cells in human cord blood. Transfusion 55:1008-12 (2015). PubMed: 25412854