Human C Reactive Protein ELISA Kit (CRP) (ab99995)
Key features and details
- Sensitivity: 2 pg/ml
- Range: 34.29 pg/ml - 25000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human C Reactive Protein ELISA Kit (CRP)
C Reactive Protein キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 2 pg/ml -
検出範囲
34.29 pg/ml - 25000 pg/ml -
添加回収試験
> 100 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 106.2 95% - 116% Serum 116.7 106% - 127% Plasma 102.2 93% - 112% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s C Reactive Protein (CRP) Human ELISA (Enzyme- Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CRP in serum, plasma, cell culture supernatants.
This assay employs an antibody specific for Human CRP coated on a 96-well plate. Standards and samples are pipetted into the wells and CRP present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CRP antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CRP bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get results in 90 minutes with Human C Reactive Protein ELISA Kit (ab181416) from our SimpleStep ELISA® range.
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特記事項
Optimization may be required with urine samples.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 2 x 15ml CRP Microplate (12 x 8 well strips) 1 unit Biotinylated anti-Human CRP 2 vials Recombinant Human CRP Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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機能
Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. -
組織特異性
Found in plasma. -
配列類似性
Belongs to the pentaxin family.
Contains 1 pentaxin domain. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Pentraxin 1, short
- C reactive protein
- C reactive protein pentraxin related
see all -
参照データベース
- Entrez Gene: 1401 Human
- Omim: 123260 Human
- SwissProt: P02741 Human
- Unigene: 709456 Human
- Unigene: 76452 Human
画像
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (33)
ab99995 は 33 報の論文で使用されています。
- Minas TZ et al. Serum proteomics links suppression of tumor immunity to ancestry and lethal prostate cancer. Nat Commun 13:1759 (2022). PubMed: 35365620
- Barcikowska Z et al. Effect of Manual Therapy Compared to Ibuprofen on Primary Dysmenorrhea in Young Women-Concentration Assessment of C-Reactive Protein, Vascular Endothelial Growth Factor, Prostaglandins and Sex Hormones. J Clin Med 11:N/A (2022). PubMed: 35628817
- Kared H et al. Immune responses in Omicron SARS-CoV-2 breakthrough infection in vaccinated adults. Nat Commun 13:4165 (2022). PubMed: 35851055
- Han D et al. Upregulated lncRNA-NEF predicts recurrence and poor treatment outcomes of ankylosing spondylitis. Immun Inflamm Dis 10:e627 (2022). PubMed: 35894706
- Ferguson DP et al. C-reactive protein and telomerase reverse transcriptase (TERT) associate with chronic disease markers in a sample from low-income neighborhoods in Detroit, Michigan. Sports Med Health Sci 4:275-279 (2022). PubMed: 36600969