Human BMP5 ELISA Kit (ab119583)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 156 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Serum, Tissue
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human BMP5 ELISA Kit
BMP5 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% 1 16 242pg/ml 16.45 = 6.8% 2 16 1573pg/ml 69.21 = 4.4% 3 16 4363pg/ml 314.13 = 7.2% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% 1 24 266pg/ml 20.74 = 7.8% 2 24 1683pg/ml 82.46 = 4.9% 3 24 4290pg/ml 347.49 = 8.1% -
サンプルの種類
Cell culture supernatant, Serum, Tissue -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 10 pg/ml -
検出範囲
156 pg/ml - 10000 pg/ml -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s Human BMP5 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human BMP5 in cell culture supernatants, serum and bone tissue.
A BMP5 specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for BMP5 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human BMP5 amount of sample captured in plate.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 ラベル 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Human BMP5 antibody Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti-Human BMP5 antibody 1 x 100µl Lyophilized recombinant Human BMP5 standard 2 x 10ng Plate Seal 1 x 4 units Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Amber Bottle 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml Wash Buffer (25X) 1 x 20ml -
研究分野
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機能
Induces cartilage and bone formation. -
組織特異性
Expressed in the lung and liver. -
配列類似性
Belongs to the TGF-beta family. -
細胞内局在
Secreted. - Information by UniProt
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別名
- BMP 5
- BMP-5
- Bmp5
see all -
参照データベース
- Entrez Gene: 653 Human
- Omim: 112265 Human
- SwissProt: P22003 Human
- Unigene: 296648 Human
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab119583 は 1 報の論文で使用されています。
- Xie S et al. Recombinant human bone morphogenetic protein 2 and 7 inhibit the degeneration of intervertebral discs by blocking the Puma-dependent apoptotic signaling. Int J Biol Sci 17:2367-2379 (2021). PubMed: 34239363