Application
Western blot
Sample
Human Cell lysate - whole cell (colon epithelial cell line)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
50 µg
Specification
colon epithelial cell line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% bsa
Dilution
1/500
Secondary antibody
Dilution
1/3000
Name
Non-Abcam antibody was used: DAKO
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
10 second(s)
Bands
Specific: 85 kDa
Positive control
HELA cells
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 24 2016