Anti-Hsp90 beta 抗体 [K3705] (ab82522)
Key features and details
- Mouse monoclonal [K3705] to Hsp90 beta
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human, Chinese hamster
- Isotype: IgM
製品の概要
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製品名
Anti-Hsp90 beta antibody [K3705]
Hsp90 beta 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [K3705] to Hsp90 beta -
由来種
Mouse -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Mouse, Rat, Human, Chinese hamster -
免疫原
Recombinant human Hsp90 beta protein
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ポジティブ・コントロール
- WB: A431, Saos-2, HL-60, HeLa, PC-12 cells, CHO-K1 cells and 3T3 cells. IHC-P: Human pancreas FFPE tissue sections.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.1% Sodium azide -
Concentration information loading...
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精製度
Ammonium Sulphate Precipitation -
ポリ/モノ
モノクローナル -
クローン名
K3705 -
アイソタイプ
IgM -
研究分野
関連製品
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Compatible Secondaries
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KO cell lines
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KO cell lysates
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab82522の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB | (1) |
1/1000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
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特記事項 |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/1000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa). |
ターゲット情報
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機能
Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. -
配列類似性
Belongs to the heat shock protein 90 family. -
ドメイン
The TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins. -
翻訳後修飾
Ubiquitinated in the presence of STUB1-UBE2D1 complex (in vitro).
ISGylated.
S-nitrosylated; negatively regulates the ATPase activity. -
細胞内局在
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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参照データベース
- Entrez Gene: 3326 Human
- Entrez Gene: 15516 Mouse
- Entrez Gene: 301252 Rat
- Omim: 140572 Human
- SwissProt: P08238 Human
- SwissProt: P11499 Mouse
- SwissProt: P34058 Rat
- Unigene: 509736 Human
see all -
別名
- 90 kda heat shock protein beta HSP90 beta antibody
- D6S182 antibody
- FLJ26984 antibody
see all
画像
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All lanes : Anti-Hsp90 beta antibody [K3705] (ab82522) at 1/1000 dilution
Lane 1 : Wild-type A-431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : HSP90AB1 knockout A-431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : Saos-2 (Human osteosarcoma cell line) whole cell lysate
Lane 4 : HL-60 (Human promyelocytic leukemia cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab82522 observed at 85 kDa. Red - loading control, ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) observed at 37kDa.
ab82522 was shown to react with Hsp90 beta in wild-type A431 cells in western blot. Loss of signal was observed when HSP90AB1 knockout sample was used. Wild-type A431 and HSP90AB1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab82522 and ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-Hsp90 beta antibody [K3705] (ab82522) at 1/1000 dilution
Lane 1 : Molecular weight markers
Lane 2 : Hsp90
Native Human Protein
Lane 3 : Hsp90 alpha Recombinant Human
Protein
Lane 4 : Hsp90 beta Recombinant Human Protein
Lane 5 : HeLa cells
Lane 6 : PC-12 cells
Lane 7 : CHO-K1 cells
Lane 8 : 3T3 cells
Predicted band size: 83 kDa
Observed band size: 83 kDa -
ab82522, at 1/50 dilution, staining Hsp90 beta in human breast cancer tissue by Immunohistochemistry.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab82522 は 4 報の論文で使用されています。
- Samson AL et al. Physicochemical properties that control protein aggregation also determine whether a protein is retained or released from necrotic cells. Open Biol 6:N/A (2016). PubMed: 27810968
- Soleilhavoup C et al. Proteomes of the Female Genital Tract During the Oestrous Cycle. Mol Cell Proteomics 15:93-108 (2016). PubMed: 26518761
- Soleilhavoup C et al. Ram seminal plasma proteome and its impact on liquid preservation of spermatozoa. J Proteomics 109C:245-260 (2014). WB ; Sheep . PubMed: 25053255
- Nemoto T et al. Domain structures and immunogenic regions of the 90-kDa heat-shock protein (HSP90). Probing with a library of anti-HSP90 monoclonal antibodies and limited proteolysis. J Biol Chem 272:26179-87 (1997). PubMed: 9334185