You have changed your country from to
. Please be aware that this will change the currency in the purchasing process.
Related products
- Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab34909 for help.
Alternatively, you can search the previous enquiries about this product to see if your query has already been answered.
|
|||||||||
 | |||||||||
|
|||||||||
We are planning to buy your new Hsp90 antibody (ab34909) for use in an ELISA set up with Drosophila melanogaster. Have you actually tested whether this antibody works on this species in an ELISA set up (or whether other antibodies - e.g. your monoclonal Hsp90 antibody would be more appropriate)? We need to be able to detect small differences and therefore need to know about detection levels, sensitivities and how specific the antibody is. Also can you suggest secondary antibodies?
|
|||||||||
ANSWER: |
Thank you for your interesting enquiry. I have looked at the details for all the antibodies we have raised against Hsp90. There is another one available, rabbit polyclonal ab13494 that will be more suitable for your application. It has been tested in ELISA and also in Drosophila. The sensitivity of the assay you develop is going to depend on the buffers, incubation conditions, substrate, etc. All antibodies will require optimisation for your specific application and conditions. However, there is a citation for this antibody in which they used a 1:100 dilution in ELISA. There is also a citation in which this antibody(ab13494) was used against drosophila samples. Hayem, G. et al (1999) Anti-heat shock protein 70 kDa and 90 kDa antibodies in serum of patients with rheumatoid arthritis. Ann Rheum Dis 58(5): 291-296 de Carcer. et al,(2001) Requirement of Hsp90 for centrosomal function reflects its regulation of Polo kinase stability. EMBO J 20(11): 2878-2884 Available secondary antibodies are: Biotinylated ab6734, ab37961 HRP ab37940, ab6734 Alk phos ab6846 I hope this helps. Please don't hesitate to get back to me if you have any more queries.
|
||||||||
|
|||||||||
| |||||||||
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
