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ab34909 has been referenced in 1 publications.
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All lanes : Anti-Hsp90 antibody (ab34909) at 1/500 dilution
Lane 1 : Cos7 cells transfected with mutant K294R Hsp90, treated with Trichostatin A (an HDAC inhibitor)
Lane 2 : Cos7 cells transfected with mutant K294R Hsp90, mock treated with DMSO only
Lane 3 : Cos7 cells transfected wild type Hp90, treated with Trichostatin A (an HDAC inhibitor)
Lane 4 : Cos7 cells transfected wild type Hp90, mock treated with DMSO only
Lane 5 : Cos 7 cells transfected with empty vector, treated with Trichostatin A (an HDAC inhibitor)
Lane 6 : Cos 7 cells transfected with empty vector, mock treated with DMSO only
Predicted band size : 95 kDa
Observed band size : 95 kDa
Transfected cells express either mutant or wt Hsp90 coupled to FlagTM tag. Anti-FlagTM immunoprecipitation was performed prior to immunoblotting with anti-Hsp90.
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