Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (4-20% gradient gel)
Sample
Rat Tissue lysate - whole (rat brain celles)
Specification
rat brain celles
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 4°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/10000
Secondary antibody
Name
Non-Abcam antibody was used: Doankey anti Rabbit frm Pierce
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
Clairity signal ECL Biorad
Bands
Specific: 90-95 kDa
Exposure
1 minute(s) and 0 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Oct 10 2014