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Immunocytochemistry/ Immunofluorescence - Hsp90 antibody (ab13495)
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Product Name
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Hsp90 antibody
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Hsp90 抗体 (17件) 一覧
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Hsp90
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Immunogen
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Human Hsp90.
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Reacts with
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Hu, Ms, Rat
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Specificity
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Detects 90kD proteins corresponding to the molecular mass of hsp90aß.
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Tested applications
(see key)
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ELISA, ICC, IHC-Fr, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: 1/20000 - 1/40000. Detects a band of approximately 90 kDa (predicted molecular weight: 83.2 kDa (beta) & 84.5 kDa (alpha) kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa Cell Lysate (Heat Shocked).
Hsp90 Protein.
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Cellular localization
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Cytoplasmic
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Research areas
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Cancer >> Tumor biomarkers >> Other
Signal Transduction >> Protein Trafficking >> Chaperones >> Heat Shock Proteins
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Relevance
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In mammalian cells there are at least two Hsp90 isoforms, Hsp90a and Hsp90b which are encoded by separate genes. These ubiquitous and highly conserved proteins account for 1-2% of all cellular proteins in most cells. Hsp90 is part of the cells powerful network of chaperones to fight the deleterious consequences of protein unfolding caused by nonphysiological conditions. However, in the absence of stress, Hsp90 is a necessary component of fundamental cellular processes such as hormone signaling and cell cycle control. In this context several key regulatory proteins such as steriod receptors, cell cycle kinases involved in signal transduction and p53 have been identified as substrates of Hsp90. It has been suggested that Hsp90 acts as a capacitor for morphological evolution by buffering widespread variation, which may affect morphogenic pathways.
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Hsp90 antibody (ab13495)の別名 |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Preservative: None
Constituents: Whole Serum
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Form
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Liquid
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Concentration
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Concentration not determined - その理由
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Abcam では全ての製品情報を一元管理しており、 Hsp90 antibody について私たちが知りうる全ての情報がこのデータシートでご覧いただけます。ご不明な点がありましたら、 お問い合わせください。 |
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See below for Hsp90 antibody images, references, products related to ab13495 and other tools.
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Hsp90 antibody images:
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 Immunocytochemistry/ Immunofluorescence - Hsp90 antibody (ab13495)
ab13495 at a 1/100 dilution staining Hsp90 in human PMN cells by Immunocytochemistry/ Immunofluorescence incubated for 4 hours at 37°C. PFA fixed. Blocked using 2% BSA for 1 hour at 22°C. Secondary used at 1/250 polyclonal Goat anti-rabbit IgG (H+L) conjugated to Alexa Fluor 568.Left image: DAPI staining nuclei (blue)Middle image: Hsp90 (red)Right image: Overlay This image was kindly supplied by Dr Mahesh Shivananjappa by Abreview
See Abreview
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Search PubMed (MEDLINE) for references to Hsp90
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Hsp90 antibody - more information
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Customer reviews (feedback) regarding Hsp90 antibody |
Customer FAQs regarding Hsp90 antibody |
Protocols for Hsp90 antibody |
Price and availability of products related to Hsp90 antibody |
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Hsp90 antibody - related products:
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Hsp90 antibody - other tools:
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Contact Abcam with a Technical Enquiry about Hsp90 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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