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Products:Signal Transduction >> Protein Trafficking >> Chaperones >> Heat Shock Proteins
Overview
Product name
Anti-Hsp90 antibody
Hsp90 抗体 (21件) 一覧
Description
Rabbit polyclonal to Hsp90
Specificity
Detects 90kD proteins corresponding to the molecular mass of hsp90aß.
Tested applications
ELISA, IP, IHC-P, IHC-Fr, ICC, Flow Cyt, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human, Xenopus laevis
Immunogen
Human Hsp90.
Positive control
HeLa Cell Lysate (Heat Shocked). Hsp90 Protein.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: Whole Serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Other
Signal Transduction >> Protein Trafficking >> Chaperones >> Heat Shock Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab13495 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
ICC: Use at an assay dependent dilution.
Flow Cyt: 1/250((see Abreview).)
WB: 1/20000 - 1/40000.Detects a band of approximately 90 kDa (predicted molecular weight: 83.2 (beta) , 84.5 (alpha) kDa).
Target
Relevance
In mammalian cells there are at least two Hsp90 isoforms, Hsp90a and Hsp90b which are encoded by separate genes. These ubiquitous and highly conserved proteins account for 1-2% of all cellular proteins in most cells. Hsp90 is part of the cells powerful network of chaperones to fight the deleterious consequences of protein unfolding caused by nonphysiological conditions. However, in the absence of stress, Hsp90 is a necessary component of fundamental cellular processes such as hormone signaling and cell cycle control. In this context several key regulatory proteins such as steriod receptors, cell cycle kinases involved in signal transduction and p53 have been identified as substrates of Hsp90. It has been suggested that Hsp90 acts as a capacitor for morphological evolution by buffering widespread variation, which may affect morphogenic pathways.
Cellular localization
Cytoplasmic
Alternative names
- D6S182 antibody
- FLJ26984 antibody
- FLJ31884 antibody
see all
Database links
- Entrez Gene: 3320 Human
- Entrez Gene: 3326 Human
- Entrez Gene: 15516 Mouse
- Entrez Gene: 15519 Mouse
- Entrez Gene: 301252 Rat
- Omim: 140571 Human
- Omim: 140572 Human
- SwissProt: P07900 Human
see all
Anti-Hsp90 antibody images:
Immunocytochemistry/ Immunofluorescence - Hsp90 antibody (ab13495)
ab13495 at a 1/100 dilution staining Hsp90 in human PMN cells by Immunocytochemistry/ Immunofluorescence incubated for 4 hours at 37°C. PFA fixed. Blocked using 2% BSA for 1 hour at 22°C. Secondary used at 1/250 polyclonal Goat anti-rabbit IgG (H+L) conjugated to Alexa Fluor 568.Left image: DAPI staining nuclei (blue)Middle image: Hsp90 (red)Right image: Overlay
This image was kindly supplied by Dr Mahesh Shivananjappa by Abreview
Flow Cytometry - Hsp90 antibody (ab13495)
ab13495 staining Hsp90 in Human platelet cells by Flow cytometry.
Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton-X-100 in 2% BSA for 15 minutes. Primary antibody used at a 1/250 dilution and incubated for 18 hours at 4°C. The secondary antibody used was an Alexa Fluor®488 conjugated chicken anti-rabbit IgG (H+L) at a 1/500 dilution.
Image courtesy of Dr Mahesh Shivananjappa by Abreview.
Western blot - Anti-Hsp90 antibody (ab13495)
Anti-Hsp90 antibody (ab13495) at 1/1000 dilution + whole cell lysate prepared from human platelets treated with A23187 for 1 hour at 20 µg
Secondary
HRP conjugated goat anti-rabbit polyclonal at 1/10000 dilution
developed using the ECL technique
Predicted band size : 83.2 (beta) , 84.5 (alpha) kDa
Observed band size : 90 kDa (why is the actual band size different from the predicted?)
Additional bands at : 36 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 5 minutes
Image courtesy of Dr Mahesh Shivananjappa by Abreview.
References for Anti-Hsp90 antibody (ab13495)
This product has been referenced in:
- Deng MYet al. Frontal-Subcortical Protein Expression following Prenatal Exposure to Maternal Inflammation. PLoS One 6:e16638 (2011). WB; Mouse.Read more (PubMed: 21347362) »
- Savulescu AFet al. Nuclear import of an intact preassembled proteasome particle. Mol Biol Cell 22:880-91 (2011). WB; Xenopus laevis.Read more (PubMed: 21289101) »
See all 2 publications for this product
Publishing research using ab13495? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"