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Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab13495? Please let us know so that we can cite the reference in this datasheet
ab13495 has been referenced in 2 publications.
- Deng MY et al. Frontal-Subcortical Protein Expression following Prenatal Exposure to Maternal Inflammation. PLoS One 6:e16638 (2011). WB; Mouse. PubMed: 21347362
- Savulescu AF et al. Nuclear import of an intact preassembled proteasome particle. Mol Biol Cell 22:880-91 (2011). WB; Xenopus laevis. PubMed: 21289101
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Immunocytochemistry/ Immunofluorescence - Hsp90 antibody (ab13495)
ab13495 at a 1/100 dilution staining Hsp90 in human PMN cells by Immunocytochemistry/ Immunofluorescence incubated for 4 hours at 37°C. PFA fixed. Blocked using 2% BSA for 1 hour at 22°C. Secondary used at 1/250 polyclonal Goat anti-rabbit IgG (H+L) conjugated to Alexa Fluor 568.Left image: DAPI staining nuclei (blue)Middle image: Hsp90 (red)Right image: Overlay
This image was kindly supplied by Dr Mahesh Shivananjappa by Abreview
Flow Cytometry - Hsp90 antibody (ab13495)
ab13495 staining Hsp90 in Human platelet cells by Flow cytometry.
Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton-X-100 in 2% BSA for 15 minutes. Primary antibody used at a 1/250 dilution and incubated for 18 hours at 4°C. The secondary antibody used was an Alexa Fluor®488 conjugated chicken anti-rabbit IgG (H+L) at a 1/500 dilution.
Image courtesy of Dr Mahesh Shivananjappa by Abreview.
Western blot - Anti-Hsp90 antibody (ab13495)
Anti-Hsp90 antibody (ab13495) at 1/1000 dilution + whole cell lysate prepared from human platelets treated with A23187 for 1 hour at 20 µg
Secondary
HRP conjugated goat anti-rabbit polyclonal at 1/10000 dilution
developed using the ECL technique
Predicted band size : 83.2 (beta) , 84.5 (alpha) kDa
Observed band size : 90 kDa (why is the actual band size different from the predicted?)
Additional bands at : 36 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 5 minutes
Image courtesy of Dr Mahesh Shivananjappa by Abreview.
Hsp90 antibody for ICC in Human (13495)
Anti-Hsp90 antibody for Immunoprecipitation in Mouse (13495)
Anti-Hsp90 antibody for Immunocytochemistry/ Immunofluorescence in Mouse (13495)
Hsp90 antibody for Flow Cytometry in Human (13495)
Anti-Hsp90 antibody for Western blot in Human (13495)
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