Hsp90 antibody [S88] (ab1429)

Product Name 

Hsp90 antibody [S88]

Product type 

Primary antibodies

Description 

Mouse monoclonal [S88] to Hsp90

Immunogen 

Full length native protein (purified) (Achlya ambisexualis).

Reacts with 
(species key)

Hu, Ms, Rat, Chk, Cow, Dog, Gpig, Mk, Pig, Rb, Shp

Specificity 

The antibody recognizes a 90 kDa protein, corresponding to the apparent molecular weight of Hsp90 on SDS-PAGE immunoblots. This antibody detects both Hsp90 alpha and beta.

Tested applications 
(see key)

IHC-Fr, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
IHC-Fr: Use at a concentration of 10 - 20 µg/ml.
IP: Use at a concentration of 4 µg/ml.
WB: Use at a concentration of 1 µg/ml.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Heat shocked HeLa cell lysate and recombinant Hsp90 protein

Cellular localization 

Cytoplasmic

Research areas 

Cancer >> Tumor biomarkers >> Other
Signal Transduction >> Protein Trafficking >> Chaperones >> Heat Shock Proteins

Relevance 

In mammalian cells there are at least two Hsp90 isoforms, Hsp90a and Hsp90b which are encoded by separate genes. These ubiquitous and highly conserved proteins account for 1-2% of all cellular proteins in most cells. Hsp90 is part of the cells powerful network of chaperones to fight the deleterious consequences of protein unfolding caused by nonphysiological conditions. However, in the absence of stress, Hsp90 is a necessary component of fundamental cellular processes such as hormone signaling and cell cycle control. In this context several key regulatory proteins such as steriod receptors, cell cycle kinases involved in signal transduction and p53 have been identified as substrates of Hsp90. It has been suggested that Hsp90 acts as a capacitor for morphological evolution by buffering widespread variation, which may affect morphogenic pathways.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

S88

Isotype 

IgG1

Storage buffer 

PBS pH7.2 with 50% glycerol, 1% BSA and 0.02% sodium azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.200 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for Hsp90 antibody [S88] images, references, products related to ab1429 and other tools.

Hsp90 antibody [S88] images:

References for Hsp90 antibody [S88] (ab1429)

  This product has been used in: (two most recent references)

Cheng J et al. 20-hydroxy-5,8,11,14-eicosatetraenoic acid mediates endothelial dysfunction via IkappaB kinase-dependent endothelial nitric-oxide synthase uncoupling. J Pharmacol Exp Ther 332:57-65 (2010). PubMed: 19841472

Wen KW & Damania B Hsp90 and Hsp40/Erdj3 are required for the expression and anti-apoptotic function of KSHV K1. Oncogene 29:3532-44 (2010). WB, IP; Human. PubMed: 20418907

See all 6 publication references for this product.


If you publish research using ab1429 please let us know so that we can cite the reference on this datasheet.

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