![Western blot - Hsp90 antibody [16F1] (ab13494) image](#ab13494_1.jpg)
![Western blot - Hsp90 antibody [16F1] (ab13494) image](#Hsp90-Primary-antibodies-ab13494-1.jpg)
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Products:Signal Transduction >> Protein Trafficking >> Chaperones >> Heat Shock Proteins
Overview
Product name
Anti-Hsp90 antibody [16F1]
Hsp90 抗体 (21件) 一覧
Description
Rat monoclonal [16F1] to Hsp90
Specificity
ab13494 detects both the alpha and beta isoforms of Hsp90.
Tested applications
IHC-P, ELISA, WB, IPmore details
Cross reactivity
Reacts with
Mouse, Rat, Sheep, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Pig, Carp, Fruit fly (Drosophila melanogaster), Monkey, Zebrafish, Rainbow Trout
Predicted to work with
Plants
Immunogen
Human Hsp90 purified from therapeutic orchiectomy specimens.
Positive control
HeLa Cell Lysate (Heat Shocked). Hsp90 Protein.
General notes
This antibody is highly recommended over other Hsp90 antibodies for use with samples of fish origin.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 50% Glycerol, PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
This product has been affinity purified.
Clonality
Monoclonal
Clone number
16F1
Isotype
IgG2a
Research areas
Cancer >> Tumor biomarkers >> Other
Signal Transduction >> Protein Trafficking >> Chaperones >> Heat Shock Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab13494 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependent dilution.
ELISA
ELISA: 1/5000 - 1/25000.ELISA: 1/5000 - 1/25000.
WB: Use a concentration of 2 µg/mlDetects a band of approximately 90 kDa (predicted molecular weight: 83.2 (beta) , 84.5 (alpha) kDa).
IP: Use at an assay dependent dilution.
Target
Relevance
In mammalian cells there are at least two Hsp90 isoforms, Hsp90a and Hsp90b which are encoded by separate genes. These ubiquitous and highly conserved proteins account for 1-2% of all cellular proteins in most cells. Hsp90 is part of the cells powerful network of chaperones to fight the deleterious consequences of protein unfolding caused by nonphysiological conditions. However, in the absence of stress, Hsp90 is a necessary component of fundamental cellular processes such as hormone signaling and cell cycle control. In this context several key regulatory proteins such as steriod receptors, cell cycle kinases involved in signal transduction and p53 have been identified as substrates of Hsp90. It has been suggested that Hsp90 acts as a capacitor for morphological evolution by buffering widespread variation, which may affect morphogenic pathways.
Cellular localization
Cytoplasmic
Alternative names
- D6S182 antibody
- FLJ26984 antibody
- FLJ31884 antibody
see all
Database links
- Entrez Gene: 396188 Chicken
- Entrez Gene: 281832 Cow
- Entrez Gene: 386588 Cow
- Entrez Gene: 3320 Human
- Entrez Gene: 3326 Human
- Entrez Gene: 15516 Mouse
- Entrez Gene: 15519 Mouse
- Entrez Gene: 396742 Pig
see all
Anti-Hsp90 antibody [16F1] images:
Western blot - Hsp90 antibody [16F1] (ab13494)
![Western blot - Hsp90 antibody [16F1] (ab13494)](/ps/datasheet/Images/13/ab13494/ab13494_1.jpg)
Lane 1 : Anti-Hsp90 antibody [16F1] (ab13494) at 1000
Lane 2 : No primary
Lane 1 : 15ug/lane HEK293 whole cell lysate
Lane 2 : 15ug/lane HEK293 whole cell lysate
Secondary
Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (AP) (ab6735) at 1/1000 dilution
Performed under reducing conditions.
Predicted band size : 83.2 (beta) , 84.5 (alpha) kDa
Observed band size : 85 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 minute
This image is courtesy of an Abreview submitted by Dr Alan Hendrick
Western blot - Hsp90 antibody [16F1] (ab13494)
![Western blot - Hsp90 antibody [16F1] (ab13494)](/ps/datasheet/images/13/ab13494/Hsp90-Primary-antibodies-ab13494-1.jpg)
All lanes : Anti-Hsp90 antibody [16F1] (ab13494) at 1/1000 dilution
Lane 1 : Molecular weight ladder
Lane 2 : Hsp90 native protein
Lane 3 : Hsp90 recombinant protein
Lane 4 : Hsp90 alpha recombinant protein
Lane 5 : heat shocked Hela cell lysates
Lane 6 : L-929 cell lysates
Lane 7 : Rat-2 cell lysates
Lane 8 : RK-13 cell lysates
Predicted band size : 83.2 (beta) , 84.5 (alpha) kDa
References for Anti-Hsp90 antibody [16F1] (ab13494)
This product has been referenced in:
- Hirano Met al. Transcriptomic analysis of rat brain tissue following gamma knife surgery: early and distinct bilateral effects in the un-irradiated striatum. Mol Cells 27:263-8 (2009). WB; Rat.Read more (PubMed: 19277511) »
- Hawkins TAet al. The ATPase-dependent chaperoning activity of Hsp90a regulates thick filament formation and integration during skeletal muscle myofibrillogenesis. Development 135:1147-56 (2008). WB; Zebrafish.Read more (PubMed: 18256191) »
See all 6 publications for this product
Publishing research using ab13494? Please let us know so that we can cite the reference in this datasheet
![Hsp90 antibody [16F1] for WB in Human (13494)](/ps/Reviews/Images/ab4736_54862.jpg)
![Hsp90 antibody [16F1] for WB in Human (13494)](/ps/Reviews/Images/ab5562_21176.jpg)
![Hsp90 antibody [16F1] for WB in Human (13494)](/ps/Reviews/Images/ab9528_47984.jpg)
![Hsp90 antibody [16F1] for Immunocytochemistry/ Immunofluorescence in Fruit fly (Drosophila melanogaster) (13494)](/ps/datasheet/images/13/ab13494/Hsp90-Primary-antibodies-ab13494-3.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"