Anti-Hormone sensitive lipase/HSL (phospho S853) 抗体 [EPR2329(2)] (ab109400)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2329(2)] to Hormone sensitive lipase/HSL (phospho S853)
- Suitable for: WB
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)]
Hormone sensitive lipase/HSL 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR2329(2)] to Hormone sensitive lipase/HSL (phospho S853) -
由来種
Rabbit -
特異性
The antibody only detects HSL phosphorylated on serine 853. -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF,IHC-P or IP -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Human skeletal muscle lysates and Mouse muscle lysates
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR2329(2) -
アイソタイプ
IgG -
研究分野
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109400の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/50000 - 1/200000. Detects a band of approximately 83 kDa (predicted molecular weight: 117 kDa).
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特記事項 |
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WB
1/50000 - 1/200000. Detects a band of approximately 83 kDa (predicted molecular weight: 117 kDa). |
ターゲット情報
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機能
In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production. -
パスウェイ
Glycerolipid metabolism; triacylglycerol degradation. -
配列類似性
Belongs to the 'GDXG' lipolytic enzyme family. -
細胞内局在
Cell membrane. Membrane > caveola. Cytoplasm > cytosol. Found in the high-density caveolae. Translocates to the cytoplasm from the caveolae upon insulin stimulation. - Information by UniProt
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参照データベース
- Entrez Gene: 3991 Human
- Entrez Gene: 16890 Mouse
- Entrez Gene: 25330 Rat
- Omim: 151750 Human
- SwissProt: Q05469 Human
- SwissProt: Q6NSL7 Human
- SwissProt: P54310 Mouse
- SwissProt: P15304 Rat
see all -
別名
- Hormone sensitive lipase antibody
- Hormone sensitive lipase testicular isoform antibody
- Hormone-sensitive lipase antibody
see all
画像
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All lanes : Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)] (ab109400) at 1/50000 dilution
Lane 1 : Human skeletal muscle lysates
Lane 2 : Human skeletal muscle lysates treated with Lambda Phosphatase
Lysates/proteins at 10 µg per lane.
Predicted band size: 117 kDa
Observed band size: 83 kDa why is the actual band size different from the predicted? -
All lanes : Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)] (ab109400) at 1/50000 dilution
Lane 1 : Mouse muscle lysate
Lane 2 : Mouse muscle lysate treated with Alkaline Phosphatase
Lysates/proteins at 10 µg per lane.
Predicted band size: 117 kDa
Observed band size: 83 kDa why is the actual band size different from the predicted?
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab109400 は 6 報の論文で使用されています。
- Yang X et al. Targeting adipocytic discoidin domain receptor 2 impedes fat gain while increasing bone mass. Cell Death Differ 29:737-749 (2022). PubMed: 34645939
- Li J et al. Purinergic 2X7 receptor is involved in adipogenesis and lipid degradation. Exp Ther Med 23:81 (2022). PubMed: 34934450
- Zhao Y et al. Expression of Recombinant Rat Secretable FNDC5 in Pichia Pastoris and Detection of Its Biological Activity. Front Endocrinol (Lausanne) 13:852015 (2022). PubMed: 35321332
- Gao LM et al. Maternal pyrimidine nucleoside supplementation regulates fatty acid, amino acid and glucose metabolism of neonatal piglets. Anim Nutr 11:309-321 (2022). PubMed: 36312745
- Wu W et al. MicroRNA-29b/29c targeting CTRP6 influences porcine adipogenesis via the AKT/PKA/MAPK Signalling pathway. Adipocyte 10:264-274 (2021). PubMed: 33938394
- Feng J et al. IL-25 stimulates M2 macrophage polarization and thereby promotes mitochondrial respiratory capacity and lipolysis in adipose tissues against obesity. Cell Mol Immunol 15:493-505 (2018). PubMed: 28194019