Application
Western blot
Sample
Human Cell lysate - whole cell (293 cells)
Loading amount
20 µg
Specification
293 cells
Treatment
siHomer1 siRNA
Gel Running Conditions
Reduced Denaturing (12 % SDS PAGE)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: TBST
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-mouse IgG-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/4000
Detection
Detection method
ECL Promega
Exposure
20 second(s)
Bands
Specific: 39 kDa
Positive control
Cells treated with control siRNA were used as control.
Additional data
Additional Notes
siRNA treatment for Homer1 doesnt seem to have been very effective, but antibody is good for WB
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jan 25 2013