Anti-Homer1 抗体 (ab97593)
Key features and details
- Rabbit polyclonal to Homer1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-Homer1 antibody
Homer1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Homer1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat, Cow -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97593の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 40 kDa.
|
IHC-P |
1/100 - 1/250.
|
特記事項 |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 40 kDa. |
IHC-P
1/100 - 1/250. |
ターゲット情報
-
機能
Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. May also couple GRM1 to PI3 kinase through its interaction with AGAP2. Isoform 1 regulates the trafficking and surface expression of GRM5. Isoform 3 acts as a natural dominant negative, in dynamic competition with constitutively expressed isoform 1 to regulate synaptic metabotropic glutamate function. Isoform 3, may be involved in the structural changes that occur at synapses during long-lasting neuronal plasticity and development. -
配列類似性
Belongs to the Homer family.
Contains 1 WH1 domain. -
ドメイン
The WH1 domain interacts with the PPXXF motif in GRM1, GRM5, RYR1, RYR2, ITPR1, SHANK 1 and SHANK3. -
細胞内局在
Cytoplasm. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell junction, synapse. Isoform 1 inhibits surface expression of GRM5 causing it to be retained in the endoplasmic reticulum. - Information by UniProt
-
参照データベース
- Entrez Gene: 9456 Human
- Entrez Gene: 29546 Rat
- Omim: 604798 Human
- SwissProt: Q86YM7 Human
- SwissProt: Q9Z214 Rat
- Unigene: 591761 Human
- Unigene: 37500 Rat
-
別名
- HOME1_HUMAN antibody
- Homer antibody
- HOMER 1A antibody
see all
画像
-
All lanes : Anti-Homer1 antibody (ab97593) at 1/1000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 40 kDa
Gel conc 10% (SDS PAGE) -
ab97593, at a 1/100 dilution, staining human Homer (1b+1c) in OVCA, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (4)
ab97593 は 4 報の論文で使用されています。
- Liang L et al. Melatonin pretreatment alleviates the long-term synaptic toxicity and dysmyelination induced by neonatal Sevoflurane exposure via MT1 receptor-mediated Wnt signaling modulation. J Pineal Res 71:e12771 (2021). PubMed: 34585785
- Lum JS et al. Effects of short- and long-term aripiprazole treatment on Group I mGluRs in the nucleus accumbens: Comparison with haloperidol. Psychiatry Res 260:152-157 (2018). PubMed: 29195167
- Matosin N et al. Molecular evidence of synaptic pathology in the CA1 region in schizophrenia. NPJ Schizophr 2:16022 (2016). WB . PubMed: 27430010
- Elagabani MN et al. Subunit-selective N-Methyl-d-aspartate (NMDA) Receptor Signaling through Brefeldin A-resistant Arf Guanine Nucleotide Exchange Factors BRAG1 and BRAG2 during Synapse Maturation. J Biol Chem 291:9105-18 (2016). PubMed: 26884337