Application
Western blot
Sample
Rat Cell lysate - nuclear (Hela cells expressing rat histone H4)
Loading amount
5 µg
Specification
Hela cells expressing rat histone H4
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL+
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 13 kDa Non-specific: 35 kDa
Additional data
Additional Notes
We used Hybond-P of GE healthcare. Image shows that wild type histone H4 but not S47A mutated histone H4 (Ser47 was changed to Ala) was recognized by this antibody.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. 克英 三宅
Verified customer
投稿 Aug 07 2009