Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (retina)
Specification
retina
Fixative
2.5% PAF + 0.1% Glu
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Other product details
Dilution
1/200
Incubation time
20 minute(s) · Temperature: 25°C · Diluent: 0.1% BSA acetylated in PBS
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabbit ultra-small
Host species: Goat
Clonality: Polyclonal
Conjugation: Gold 1.4nm (ultra-small)
Host species: Goat
Clonality: Polyclonal
Conjugation: Gold 1.4nm (ultra-small)
Dilution
1/25
Additional data
PubMed ID
PMID: 20543957: View
Additional Notes
Immunogold labeling of ultra-thin retina cryo-sections with the Tokuyasu method.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Caroline Kizilyaprak
Verified customer
投稿 Aug 26 2010