Application
ChIP
Sample
Human Cell lysate - nuclear (Colon cancer cell)
Negative control
IgG control
Specification
Colon cancer cell
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Additional data
Additional Notes
I have used this antibody as control in my experiment. good enrichment was observed at my promoter of interest as compared to IgG control. Undilluted 5 ul antibody was used for each IP. The ChIP-IT kit from Active motif is used conduct IP and ChIP.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. santosh chauhan
Verified customer
投稿 Aug 02 2011