Application
Western blot
Sample
Fruit fly (Drosophila melanogaster) Tissue lysate - nuclear (whole fruit fly)
Gel Running Conditions
Reduced Denaturing
Loading amount
4.5 µg
Specification
whole fruit fly
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS + 0.1% Tween 20 + 5% BSA
Concentration
3 µg/ml
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Anti-Rabbit IgG (H+L) Secondary Antibody, HRP conj
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 15 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Alexander Hambly
Verified customer
投稿 Apr 14 2015