Application
Western blot
Sample
Saccharomyces cerevisiae Cell lysate - whole cell (no)
Loading amount
0.5 µg
Specification
no
Treatment
125J UV treated
Gel Running Conditions
Non-reduced Denaturing (15% SDS gel)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/500
Incubation time
1 hour(s) and 30 minute(s) · Temperature: 25°C · Diluent: TBST
Secondary antibody
Name
Non-Abcam antibody was used: Biorad goat anti-rabbit IgG (H+L) HRP conjugate
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/3000
Detection
Detection method
ECL+
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: I don't know which b kDa Non-specific: 40 (Strong), 19, 17 kDa
Positive control
no positive control
Negative control
no negative control
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Peng Mao
Verified customer
投稿 Apr 29 2013