Application
Immunocytochemistry
Sample
Human Cell (HeLa)
Specification
HeLa
Permeabilization
Yes - 0.5% Triton X100 in PBS
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS
Dilution
1/500
Secondary antibody
Name
Abcam antibody:Goat Anti-Rat IgG H&L (Alexa Fluor 488)
Additional data
Additional Notes
Asynchronous cells were paraformaldehyde-fixed, permeablized, immuno-fluorescently labeled and counterstained with DAPI. This antibody produces the expected mitotic staining pattern under these conditions. MeOH-fixed samples were also evaluate and also produce the expected staining pattern.
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投稿 Mar 27 2014