Application
Immunocytochemistry/ Immunofluorescence
Blocking step
1% serum, 0.1% triton, 0.1% BSA in PBS as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
Sample
Human Cell (human neural progenitor cells from ips cell line)
Specification
human neural progenitor cells from ips cell line
Permeabilization
Yes - see blocking buffer
Fixative
Paraformaldehyde
Other product details
Concentration
2 µg/ml
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking buffer
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Rat IgG2a Alexa 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500
Additional data
Additional Notes
Following fixation in 4% PFA, the cells were assessed for Histone H3 (phospho S28) expression using primary antibody (2ug/ml) in 1% serum, 0.1% triton, 0.1% BSA in PBS. This was followed by detection using a goat polyclonal rat IgG2a Alexa 488 (1:500). Total cells were stained using DAPI (blue).
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投稿 Jan 27 2014