Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (embryonic brain section)
Specification
embryonic brain section
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
Yes - 0.1% Triton-X
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Other product details
Dilution
1/2000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS + 0.1% Triton-X + 1% normal goat ser
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-mouse IgG alexa 568
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Dilution
1/500
Additional data
Additional Notes
The primary concentration has not been optimized yet. 1:2,000 is the lowest conc. I ran in the initial serial dilution test. My guess is it can go further to 1:5,000.
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投稿 Jun 17 2008