Application
Western blot
Sample
Human Cell lysate - nuclear (rhabdomyosarcoma cell lines)
Loading amount
25 µg
Specification
rhabdomyosarcoma cell lines
Gel Running Conditions
Reduced Denaturing (10-20% tris glycine)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Dilution
1/10000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in TBST
Secondary antibody
Name
Non-Abcam antibody was used: Bio Rad secondary
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Exposure
20 second(s)
Bands
Specific: 17 kDa
Positive control
n/a
Negative control
n/a
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jan 30 2013