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Western blot - Histone H3 (di methyl K9) antibody [mAbcam 1220] (ab1220)
Western blot - Histone H3 (di methyl K9) antibody [mAbcam 1220] (ab1220)
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ChIP - Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade (ab1220)
ChIP - Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade (ab1220)
(enlarge)


Immunocytochemistry/ Immunofluorescence - Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade (ab1220)
Immunocytochemistry/ Immunofluorescence - Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade (ab1220)
(enlarge)


Product Name 

Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade

 

Histone H3 (methylated) 抗体 (36件) 一覧

Product type 

Primary antibodies

Description 

Mouse monoclonal [mAbcam 1220] to Histone H3 (di methyl K9) - ChIP Grade

Immunogen 

Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Histone H3, di methylated at K9.

.

Hybridomas were prepared and the resulting clones were positively screened by ELISA against the immunising peptide conjugated to BSA. Clones were negatively screened against both the non-modified equivalent peptide and against a peptide corresponding to dimethylated K27.

Reacts with 

Hu, Ms, Rat, At, Ce, Chk, Corn, Cow, Dm, Rice, S pom, Xl

Predicted to react 
(species key)

Sc, Shp
(Due to sequence homology)

Specificity 

By peptide ELISA ab1220 recognises di methyl K9, but not unmodified K9, mono methyl K9, tri methyl K9, di methyl K27, tri methyl K27, mono methyl K4, di methyl K4 or tri methyl K4. By Western blot ab1220 is blocked by di methyl K9, but not by unmodified K9, mono methyl K9, tri methyl K9, di methyl K27, tri methyl K27, mono methyl K4, di methyl K4 or tri methyl K4. This indicates the specificity of ab1220 for di methyl K9 of Histone H3.

Tested applications 
(see key)

ChIP, Flow Cyt, ICC/IF, IHC-P, IP, WB


Abreviews 

Customer reviews
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• ChIP

5 Abreviews

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• Flow Cyt

1 Abreview

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• ICC/IF

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• IHC-P

3 Abreviews

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• IP

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• WB

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WB
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ChIP
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WB
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WB
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Application notes 
(see key)

Recommended dilutions
ChIP: Use 1-4µg for 106 cells.
Flow Cyt: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa). Can be blocked with Histone H3 peptide - di methyl K9 (ab1772).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Chromatin and Nuclear Signaling >> Histones >> H3 >> Methylated

Relevance 

Histones are basic nuclear proteins that, together with DNA, make up the nucleosome structure in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer, which is composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin structure is further compacted through the interaction of a linker histone H1.

Methylation of specific residues in the histone tails is a central modification for regulating epigenetic transitions in chromatin. Modified lysine residues can exist in a mono-, di-, or tri-methylated state, whilst arginine residues can be mono- or di-methylated.

 

Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade (ab1220)の別名

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

176359    (Caenorhabditis elegans)
326601    (Cow)
8350    (Human)

Omim

    

601128    (Human)

SwissProt

    

P59226    (Arabidopsis thaliana)
P08898    (Caenorhabditis elegans)
Q10453    (Caenorhabditis elegans)
P68432    (Cow)
P02299    (Fruit fly (Drosophila melanogaster))
P06351    (Fruit fly (Drosophila melanogaster))
Q93081    (Human)
Q16695    (Human)
P06351    (Rat)
P02303    (Saccharomyces cerevisiae)
P16105    (Xenopus laevis)

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

mAbcam 1220

Isotype 

IgG2a

Light chain type 

kappa

Purity 

IgG fraction

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: PBS, pH 7.5

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

この製品は、毒物及び劇物取締法に基づく「毒物」に該当します。

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

Abcam では全ての製品情報を一元管理しており、 Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade について私たちが知りうる全ての情報がこのデータシートでご覧いただけます。ご不明な点がありましたら、 お問い合わせください。


See below for Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade images, references, products related to ab1220 and other tools.

 

Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade images:

Product image  Western blot - Histone H3 (di methyl K9) antibody [mAbcam 1220] (ab1220)

Western blot - Histone H3 (di methyl K9) antibody [mAbcam 1220] (ab1220)

All lanes : Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade (ab1220)

Lane 1 : Calf thymus histone lysate
Lane 2 : Calf thymus histone lysate with Histone H3 peptide - unmodified at 1 µg/ml
Lane 3 : Calf thymus histone lysate with Histone H3 peptide - mono methyl K9 (ab1771) at 1 µg/ml
Lane 4 : Calf thymus histone lysate with Histone H3 peptide - di methyl K9 (ab1772) at 1 µg/ml
Lane 5 : Calf thymus histone lysate with Histone H3 peptide - tri methyl K9 (ab1773) at 1 µg/ml
Lane 6 : Calf thymus histone lysate with Histone H3 peptide - di methyl K4 (ab7768) at 1 µg/ml
Lane 7 : Calf thymus histone lysate with Histone H3 peptide - di methyl K27 (ab1781) at 1 µg/ml

Secondary
Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab6728) at 1/5000 dilution
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 17 kDa


Exposure time : 1 minute

 

Product image  ChIP - Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade (ab1220)

ChIP - Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade (ab1220)

Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The  ChIP was performed with 25µg of chromatin, 2µg of  ab1220 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first Kb of the transcribed region.
 

 

Product image  Immunocytochemistry/ Immunofluorescence - Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade (ab1220)

Immunocytochemistry/ Immunofluorescence - Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade (ab1220)

Interphase HeLa cells were fixed in paraformaldehyde and labelled with ab1220 (1/500). The staining is shown in green. The cells were counterstained with DAPI (red).

This image was submitted as part of a review by Kirk McManus

 

Product image  - Histone H3 (di methyl K9) antibody [mAbcam 1220] (ab1220)

- Histone H3 (di methyl K9) antibody [mAbcam 1220] (ab1220)

ELISA using ab1220 at varying antibody concentrations.

The purple line indicates binding to the di methyl K9 peptide ab1772. Binding to the following peptides was not seen:
unmodified K9 (ab2903),
mono methyl K9 (ab1771),
tri methyl K9 (ab1773),
di methyl K27 (ab1781),
tri methyl K27 peptide (ab1782),
mono methyl K4 (ab1340),
di methyl K4 (ab7768),
tri methyl K4 (ab1342).

This indicates the specificity of ab1220 for di methyl K9 of Histone H3.

 

Product image  Flow Cytometry - Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade (ab1220)

Flow Cytometry - Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade (ab1220)

ab1220 staining mouse embryonic stem cells by flow cytometry (gated on all living cells). The cells were trypsinized and stained with the antibody at 1ug/1.5 x 105 cells in a permeabilization buffer. A Cy3 ® conjugated goat anti-mouse antibody was used as the secondary.

This image is courtesy of an Abreview submitted by Prof Albrecht Müller

See Abreview

 

Product image  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade (ab1220)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade (ab1220)

ab1220 staining rat liver tissue sections by IHC-P.  Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer pH 6.0 prior to blocking with 5% serum for 30 minutes at 20°C.  The primary antibody was diluted 1/400 and incubated with the sample for 45 minutes at 20°C.  A HRP-conjugated goat anti-mouse antibody was used as the secondary.

This image is courtesy of an anonymous Abreview

See Abreview

 

References for Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade (ab1220)

Specific publication references   This product has been used in: (two most recent references)

Emmerth S et al. Nuclear Retention of Fission Yeast Dicer Is a Prerequisite for RNAi-Mediated Heterochromatin Assembly. Dev Cell 18:102-113 (2010). ChIP; Schizosaccharomyces pombe. PubMed: 20152181

Bernard P et al. Splicing factor Spf30 assists exosome-mediated gene silencing in fission yeast. Mol Cell Biol 30:1145-57 (2010). ChIP. PubMed: 20028739


See all 51 publication references for this product.


If you publish research using ab1220 please let us know so that we can cite the reference on this datasheet.

 

Search PubMed (MEDLINE) for references to Histone H3

 

Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade - more information

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Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade - related products:

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Immunizing Peptide (Blocking) for Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade

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Corresponding Unmodified Peptide for Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade

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Mouse monoclonal [mAbcam 1220] to Histone H3 (di methyl K9) (FITC)

 

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Rat monoclonal [SB84a] Secondary Antibody to Mouse IgG2a - gamma chain

 

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