Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (293 cells)
Specification
293 cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 1% SDS
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Other product details
Dilution
1/200
Incubation time
1 hour(s) and 0 minute(s) · Diluent: PBS + 1% BSA
Secondary antibody
Name
Non-Abcam antibody was used: Alexa 594 anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Additional data
Additional Notes
1% SDS treatment as permeabilization and antigen retrieval step is critical (5 min).
PLF (0.2 w/v% periodate -1.4 w/v % lysine- 2% formaldehyde) as fixative also works.
PLF (0.2 w/v% periodate -1.4 w/v % lysine- 2% formaldehyde) as fixative also works.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Kathrin Bernt
Verified customer
投稿 Dec 11 2008