Application
Western blot
Loading amount
3e+007 cells
Gel Running Conditions
Non-reduced Denaturing (20% SDS-PAGE)
Sample
Plasmodium falciparum Cell lysate - whole cell (intraerythrocitic stages, synchronized culture)
Specification
intraerythrocitic stages, synchronized culture
Blocking step
Pierce Protein-Free T20 Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Pierce Protein-Free T20 Blocking Buffer
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Mouse IgG (H+L) Secondary Antibody, DyLi
Host species: Goat
Clonality: Polyclonal
Conjugation: DyLight 488
Host species: Goat
Clonality: Polyclonal
Conjugation: DyLight 488
Dilution
1/5000
Detection
Detection method
fluorescence
Bands
Specific: ca. 16 kDa Non-specific: ca. 12 kDa
Exposure
5 second(s)
Additional data
Additional Notes
Lane 1: Ring Stage
Lane 2: Trophozoite
Lane 3: Schizont
Nitrocellulose membrane
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Mar 30 2015