Application
Western blot
Sample
Ferret Tissue lysate - whole (Brain tissue)
Gel Running Conditions
Reduced Denaturing (10% Bis-Tris gel, Invitrogen)
Loading amount
100 µg
Treatment
100-200 nM of Trichostatin A (TSA), HDAC inhibitor for 24 hours
Specification
Brain tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1X TBS + 0.01% Tween + 5% non-fat milk
Dilution
1/1000
Secondary antibody
Dilution
1/4000
Name
Non-Abcam antibody was used: HRP goat anti-rabbit, Invitrogen
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Detection
Detection method
Clarity max Western ECL substrate, Bio-R
Exposure
30 second(s)
Bands
Specific: 17 kDa
Positive control
We treated ferret brain slices with Trichostatin A, TSA, a Histone deacetylase inhibitor, for 24 hours and as expected we see strong H3K9 bands with our treatment groups (2nd and 3rd lanes). TSA treatment is supposed to block deacetylation and increase acetylation as seen in the Western blot. The first lane had no treatment and served as our control. This antibody works great in ferrets.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Francis Djankpa
Verified customer
投稿 Feb 01 2017