Application
ChIP
Sample
Northern White-cheeked gibbon Cell lysate - nuclear (EBV-transformed lymphocytes)
Negative control
ChIP-seq results with validated H3K27me3 antibody (marker for gene repression)
Specification
EBV-transformed lymphocytes
Detection step
Other
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehye
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehye
Positive control
ChIP-seq results with validated H3K4me3 antibody (marker for active promoter)
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Additional data
Additional Notes
ChIP was performed using traditional home-brew protocols. Library prep was performed using NEB Ultra kit. ChIP and 10% INPUT Libraries were sequenced on the 1X100SE HiSeq platform. Reads were prep-processed and aligned to the gibbon genome using bwa-mem with default settings. Fold enrichment tracks (normalized to INPUT) were generated by MACS2. ChIP-seq tracks for active marks: H3K27ac (red track) and H3K4me3 (green track) show enrichment at the gibbon GAPHD gene promoter, while the repressive mark H3K27me3 (blue), is completely absent.
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投稿 Jan 12 2018