Application
Western blot
Loading amount
5 µg
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (12% SDS gel)
Sample
Fruit fly (Drosophila melanogaster) Tissue lysate - whole (Whole mosquito (Culex pipiens))
Specification
Whole mosquito (Culex pipiens)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 0.2% Tween 20 (TBST)
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit-Ig HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
LumiGLO chemiluminescent
Bands
Specific: 17 kDa
Exposure
10 minute(s) and 0 second(s)
Negative control
anti-GAPDH (loading control)
Positive control
Culex pipiens (whole tissues)
Additional data
Additional Notes
I have used this antibody to new organism-mosquito, Culex pipiens.
I could detect clear one band, which is ~17 Kda.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 May 30 2013